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Pintail is a tool for identifying anomalies within 16S rRNA sequences.  In essence, the program works by comparing evolutionary distances between a query and subject sequence over the entire length of the 16S rRNA gene, by employing a sampling window of specified size, progressing a fixed number of bases at a time along the length of the gene.  When two reliable (i.e., non-anomalous) 16S rRNA sequences are compared in this way, they should vary by roughly the same amount over the length of the gene.  If, however, one of the sequences is in some way anomalous (for example, chimeric), the percentage of base mismatches, along the length of the gene, will vary to a degree dependentant on the nature of the anomaly.

About Pintail

GNU General Public Licence

Valid IUB Nucleotide Base Codes

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At least one in twenty 16S rRNA sequence records currently held



At least one in twenty 16S rRNA sequence records currently held