Restrictases.

Gene Studio can mark restriction sites on the DNA molecule. Despite other features, restriction sites, when selected, cause cursor to be put at the site of cleavage. If holding Shift to click on another restriction site, the sequence between cut sites of these two restrictases will be selected in the clockwise direction.

Selecting restrictases. The set of restrictases currently marked can be seen through menu Analysis>Restriction>Select restrictases. In the opened window in right list box the are listed restrictases that are currently shown, in the left list box there are the rest of restrictases which can be selected by user. To add restrictases to the list of currently shown, select it on the left list (multiple selection allowed) and press Add button. To remove restrictase from the right list select it and press Remove button. Note that if custom restriction enzyme, added by user (see below) was removed, it will not be listed in the left list and information about it will be lost. To submit changes press "OK" button.

Creating custom restrictases. If the restrictase of interest is not in the list, user can create custom restrictase. It can be done through menu Analysis>Restriction>User restrictases. In the appeared window in the list box at the left all currently shown restrictases are listed. To add restrictase, print its name, sequence of recognition and cut positions at the direct and reverse strands, then press Add button. Add will submit new restrictase. To delete restrictase, select it in the list and press "Delete". Note that restrictases added by user will be deleted permanently, but restrictase which goes with Gene Studio can be assessed through Analysis>Restriction>Select restrictases.



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