Find Oligonucleotides: How It Works

1) The user identifies which sequence records are to be used in the design process....



2) Selected sequences are aligned (if not already)...



3) Consensus sequence generated from alignment...


4) Oligonucleotides generated from consensus...




5) Oligonucleotides sorted according to number of degenerate (i.e., non-canonical) bases...


Number of degenerate bases:
0
1
2
3
4
Oligo 1
Oligo 7
Oligo 8
Oligo 9
Oligo 12
Oligo 2


Oligo 10
Oligo 13
Oligo 3


Oligo 11
Oligo 14
Oligo 4




Oligo 5




Oligo 6




Oligo 15




Oligo 16




Oligo 17




Oligo 18




Oligo 19




Total
11
1
1
3
3