Find Oligonucleotides: Tips
- Potentially useful oligonucleotides are generated from
representatives of your target taxon. It's generally better to
select a few well chosen representatives (<15) than to select a
large number, of variable quality.
- Where possible, select type strains or other similarly reliable
sequences.
- Obviously, long sequences are preferable to small sequences.
- Aim to generate a small number of oligonucleotides (certainly no
more than 1000). The fewer number of oligonucleotides, the faster
subsequent parts of the program will be. You can always
go back and amend parameters later if none of your oligos are suitable.