Individual oligonucleotides can be explored further with this dialog
box.
Clicking the Search Database Records button causes the displayed
oligonucleotide to be compared with the displayed database.
Successful matches between oligonucleotide and sequence record are
recorded and the results displayed in various formats (see
below). A successful match can contain 0 to 7 mismatches
according to the user's selection.
Results are displayed as follows:
A graph summarises the impact of increasing allowed mismatch
number on the oligos ability to hit target records to the exclusion of
all non-target sequences.
Individual database records are colour
coded according to search
result - allowing the user to identify which records are hit by the
oligonucleotide both within and outside the target group.
the table of the top left of the dialog box lists how many
mismatches are required for a hit along with the location of the hit
within the sequence.
Graph data can be viewed by clicking the '
Display graph data'
icon. Graph itself can be printed
or saved
for future reference.
Records can be selected / deselected prior to search. Searching
disables this feature. To re-enable, click '
Enable Record Selection' - note that this will clear results.